Canine and Feline T4 Procedures at AVL

T4 measurement is a commonly requested test in veterinary medicine. T4 analysis is frequently a part of a comprehensive diagnostic panel as well as geriatric/wellness panels. T4 measurement is also frequently requested alone to monitor hypothyroidism in dogs and hyperthyroidism in cats.

The AVL Advantage!

At AVL we work to provide the most accurate testing in all areas of our laboratory, endocrinology and T4 measurements are no exception. Unlike other commercial veterinary laboratories using the same human-based reagent for the measurement of both canine and feline T4, AVL uses separate T4 reagents for the accurate measurement of circulating thyroxine in canine and feline serum.

The methodology for T4 measurement performed at AVL will also be found at most veterinary teaching hospital laboratories including: University of MO, University of IL, Cornell and Colorado State.

Methodology: Canine T4 Immulite chemiluminescent immunoassay

Each canine T4 is performed using a barcode-labeled test unit for Canine T4 containing a bead-coated monoclonal anti-T4 antibody. The reportable range is 0.5-15 ug/dl, providing accurate vales at very low levels of circulating thyroxine.

Methodology: Feline T4 Immulite chemiluminescent immunoassay

Total quantative T4 test. Unlike canine samples where linearity is important at the low end, with feline serum linearity is important at the high end. The linear reportable range for Total T4 used for cats at AVL is 1.0-24.0 ug/dl.

Quality Assurance

AVL uses a specific assayed bi-level canine serum control for our canine T4. The feline T4 assay uses a commercially available T4 control for Total T4.