Microbiology Testing

Microbiology testing is increasingly important with the emergence of resistant strains of bacteria. Microbiology analysis provides the veterinarian with a detailed diagnostic approach to treating bacterial infections. To know the genus and species of a bacterium and the sensitivity of that organism to a specific antibiotic(s), increases the efficacy of treatment, especially in chronic cases, and helps prevents antibiotic resistance.

AVL is local and can easily obtain samples directly from clinics and begin the plating process within a short time of collection. AVL is the only local laboratory with this capability.

With our new microbial identification system and MIC instrumentation we can provide an excellent turnaround time of culture results, in most cases within 24-48 hours.

MRSA

AVL screens all coagulase positive staphylococcus for MRSA using specific chromogenic media, MRSA positive samples turn a bright ruby red color. In addition, AVL also routinely drops a Cefoxitin disc on all staphylococcus isolates to detect methicillin resistance.

Anaerobic Cultures

AVL now processes anaerobic cultures in our laboratory. We use anaerobic broth enrichment procedures and pre reduced anaerobic media to provide rapid, accurate turnaround of anaerobic submissions.

 

Tests Offered

Test Description: Aerobic Culture & Sensitivity with MIC
Test Code: 336
Specimens: Swab specimens should be submitted in Amies transport or any commercial swab transport system, prefer swabs without charcoal. Submit at least one swab per culture requested. Body fluids or tissues should be submitted in a sterile, leak-proof container without preservatives. LABEL SOURCE, SPECIES, AND IF PATIENT IS CURRENTLY ON ANTIBIOTICS OR HAS BEEN IN THE LAST 2 WEEKS.
Storage: Refrigerate
Method: Specimens are plated on selective and non-selective media, enrichment procedures may also be used depending upon source of specimen. Sensitivity by disc/Kirby Bauer method on isolates
Availability: Final results available in 3-5 days

Test Description: Anaerobic Culture
Test Code: 452
Specimens: Aspirate materials into a WTT (pierce the rubber top with needle and allow vacuum to pull sample in. Do not aerate the tube by removing the top). Submit tissue in a sterile, leak-proof container or any commercial semi-solid transport media. After swabbing area to be cultured, swab should be immediately placed back into culturette.
Storage: Room Temperature
Method: Specimens are enriched in anaerobic broth and then plated anaerobically
Information:  If submitting Aerobic and Anaerobic cultures, please submit two sets of material for culture
Availability: Final results available in 7 days

Test Description: Aerobic Culture & Sensitivity with MIC + Anaerobic Culture
Test Code: 337
Specimens: See above for specific specimens. Please submit two sets of material for this test
Storage: Refrigerate
Method: Specimens are plated on selective and non-selective media, enrichment procedures may also be used depending upon source of specimen. Sensitivity by disc/Kirby Bauer method on isolates
Availability: Final results available in 3-7 days.

Test Description: Culture with ID only
Test Code: 335
Specimens: Swab specimens should be submitted in Amies transport or any commercial swab transport system. Submit at least one swab per culture requested. Body fluids or tissues should be submitted in a sterile, leak-proof container without preservatives. LABEL SOURCE, SPECIES, AND IF PATIENT IS CURRENTLY ON ANTIBIOTICS OR HAS BEEN IN THE LAST 2 WEEKS.
Storage: Refrigerate
Method: Specimens are plated on selective and non-selective media, enrichment procedures may also be used depending upon source of specimen.
Availability: Final results available in 3-5 days

Test Description: Blood Culture
Test Code: 332
Specimens: For animals weighing < or = 200 lbs, draw two lithium heparin tubes (GTT). For animals weighing > 200 lbs, draw four lithium heparin tubes (GTT). Use surgical preparation procedures on animal and GTT.
Minimum: Blood Culture Bottle or Tube (Call Lab)
Storage: Room Temperature, NEVER REFRIGERATE
Availability: Final results available in 5 days

Test Description: Direct Fecal Smear
Test Code: 206
Specimens: 5g feces in sterile fecal collection container (Fecal container/WTT)
Minimum:  3-5 grams of fresh feces
Storage: Room Temperature
Method: Microscopic evaluation
Availability: Results available daily

Test Description: Gram Stain
Test Code: 355
Specimens: One air-dried smear, swab, or fluid and tissue in a sterile, leak-proof container without preservative. Label with owner’s last name, animal’s name, and date.
Storage: Room Temperature
Availability: Performed daily with results available next day

Test Description: Fecal Pathogens
Salmonella, Shigella, & Campylobacter
Test Code: 650
Specimens: Swab or feces in a sterile collection container (WTT/Fecal Container)
Storage: Refrigerate
Method: Specimens are plated on highly selective media for isolation of fecal pathogens
Availability: Final results available in 3-4 days

Test Description: Fungal Culture
Test Code: 334
Specimens: Hair, skin, tissues, and/or nails from infected area in sterile plain tube (RTT/WTT)
Storage: Refrigerate (tissues/swabs) or Room temperature (hair, skin scrapings, nails)
Method: Specimens plated on selective media for the isolation of fungi
Information: (See test procedure section)
Availability: Set up daily. Cultures held 4 weeks before final reporting

Test Description: Fungal Identification
Test Code: 525
Specimens: Pure culture of a yeast or filamentous fungus on an agar slant in a tightly sealed tube, or a DTM tube preincubated and positive for a fungus to be identified. For primary cultures of clinical samples, see Fungal Culture, in general section.
Storage: Room Temperature
Method: Stained smears of fungal culture identified by microscopy
Availability: Results available in 2-5 days

Test Description: Fungal Serology
Aspergillus, Blastomyces, Coccidioides, and Histoplasma                
Test Code: 121
Specimens: 2mL serum (SST)
Storage: Refrigerate
Information: (See test procedure section)
Method: Immunodiffusion
Availability: Results available in 2-5 days

Test Description: Urinalysis with Culture with MIC
Test Code: 393C
Specimens: 3mL urine (WTT)
Minimum: 2mL urine (WTT)
Storage: Refrigerate
Method: Microscopy, Clinitek
Availability: Daily with culture results to follow in 3-5 days

Test Description: Urine Culture with MIC
Test Code: 326
Specimens: 1mL urine (WTT)
Minimum: 1mL urine (WTT)
Storage: Refrigerate
Availability: Daily with culture results to follow in 3-5 days